Sladjana Samuilov

5 Questions 4 Answers 0 Followers

Questions related from Sladjana Samuilov

Does slight upregulation of some genes can lead to accumulation of specific compounds?

Hi, I am working on sulfate metabolism wit Arabidopsis plant and its GDC knock-down mutant. I have a huge list of RNA-Seq results of genes involved in sulfur reduction and assimilation. Some...

08 August 2017 2,404 3 View

Does vacuum infiltration of whole leave can negatively influence the gene expression in leaves?

Leaves were catted on one side and infiltrated in exsiccator by pump about 3 min long. Thanks for your help! S.

12 December 2016 4,521 7 View

High CP values and multiple melting peaks occur by testing different primers concentrations

I have to test different primers concentration for Arabidopsis thaliana genes and its mutant by RT-qPCR. Used primers concentrations are 8µM, 10µM 12µM and 14µM. For template I used 2µL of cDNA...

11 November 2016 1,297 3 View

High CP values and multiple melting peaks occur by testing different primers concentrations

I have to test different primers concentration for Arabidopsis thaliana genes and its mutant by RT-qPCR. I made 4 different stock primers concentration: 800nM, 1000nM 1200nM and 1400nM. In final...

11 November 2016 8,831 6 View

Any suggestions for a total extracted protein content from Arabidopsis leaves?

Dear coleagues, I need to analyse total protein content in Arabidopsis leaves and I have very less leaf material. I haven`t find so many works where, specifically, protein content was analysed...

12 December 2015 7,873 9 View