What is the recommended gRNA length for knock-out of gene in Arabidopsis using CRISPR/Cas9?

I am attempting to knock-out the gene F3'H in Arabidopsis (NC_003076.8). What is the recommended length of the gRNA? I understand that it should be 17-20nt long (+PAM), but which length will...

09 September 2016 5,663 5 View