Siyuan Wang

2 Questions 4 Answers 0 Followers

Questions related from Siyuan Wang

What is the centrifuge conditions used for centrifuging lentivirus?

Hi, everyone! Recently I'm collecting lentivirus. After package in 293T cells, I take the medium, centrifuge at 1000rpm for 5 min to pellet the cells, then take the supernatant and filter by...

19 February 2017 7,304 5 View

How to prevent mES differentiation during in vitro culturing?

Hi everyone, I'm culturing mES recently, and I have found that my mES differentiate quite quickly, often on the second passage off MEF feeder. Could anyone with experience in preventing mES...

19 February 2017 4,230 2 View