Siyuan Su

10 Questions 23 Answers 0 Followers

Questions related from Siyuan Su

How should I change my Mascot identification settings in iTRAQ and TMT?

Hi, I am using Q-Exactive to quantify the BSA peptides labelled iTRAQ and TMT, separately. I checked the iTRAQ modification setting in Mascot, it assumes some of the reagent would retain on the...

04 August 2014 4,733 3 View

Is acridine orange a proper marker for lysosomes?

My purpose was to demonstrate the lysosomal permeabilization using acridine orange. I read a few papers on the LMP, most of them suggested the red fluorescence of acridine orange is an indicator...

23 February 2014 3,863 2 View

Can we export all the measured data (FCS, SSC, FITC, TRITC) for a single cell to the excel table?

I am using an Aria from BD. I want to export all the data for all the single cells to excel or txt for data analysis, not just the histogram or scatterplot. Is it possible? How to do that?

21 February 2014 565 1 View

Can anyone help with problems with statistical tests for detecting ROS using H2-DCFDA in flowcytometry?

I am trying to use flowcytometry to detect ROS using H2-DCF. Previously we have detected and compared the ROS level of treated and control cells using 96-well plate and the results are good, both...

25 November 2013 3,950 7 View

How can I reduce cytotoxicity during transfection?

I am trying to transfect the gene of interest into HeLa cells using Lipofetamine 2000 to see if this gene would cause any changes in senescence ratio (using Senescence Associated beta-gal...

28 October 2013 9,309 4 View

Is it possible for cellular senescence to occur without apparent cell cycle arrest?

Recently I checked the beta-gal staining status of my cells and found it significantly higher than in control cells. However, the cell cycle analysis using flow cytometer suggest there were no...

12 October 2013 7,108 7 View

Can someone advise on whether to perform RNase first or RNase together with PI?

I am going to do DNA content analysis on HeLa cells and I searched for relevant protocols. Some of them suggest do PI staining and RNase A together, while others stated it was better to do RNase A...

11 October 2013 4,295 3 View

Is there any reliable software that can count cells based on image taken under a microscope?

Need to process large quantities of image. Please give me some advices. Thanks

12 July 2013 2,022 22 View

Artificial effect of doxycycline on protein oxidation and transcription factors

In our lab, we often use doxycycline to induce gene expression or knockdown whose expression was regulated by Tet-regulated systems. This system is quite powerful and efficient until I found...

14 January 2013 5,431 5 View

The storage of DCF for measurement of ROS?

1. How do I make small aliquots while preventing oxidation? 2. How do I store them after I make aliquots? I read on the manual (from Molecular Probes), they say it should be stored under argon or...

26 November 2012 9,333 28 View