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Questions related from Siva Ramakrishna Uppalapati
Hi, Does anyone have experience in RNA editing with CRISPR Cas9 method? Does this tool have application limited only to gene editing or I am wondering whether intergenic regions that code for...
04 April 2018 6,507 3 View
Is it necessary to quantify RNA every time before cDNA conversion or can we just quantify cDNA after reverse transcription? How much difference does it make in relative quantification experiments?
11 November 2012 8,457 8 View
I have recently cloned and expressed an enzyme which had two domains and the recombinant protein retained its activity. When the two protein domains were cloned separately and expressed, none of...
10 October 2012 9,147 89 View
I'm standardizing a real-time duplex PCR using Power Sybr green master mix (Applied biosystems). The Tm values of my 2 amplicons; 123 bp and 213 bp are 70 and 75 C respectively. The primers have...
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