Siva Palepu

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Questions related from Siva Palepu

How to pufiry monomeric form of protein when it's in dimeric purified form?

I have purified an intrinsically disordered protein (IDP), and upon running Native PAGE, I observed that it predominantly exists in a dimeric form. I would like to isolate and purify the protein...

18 June 2025 4,943 3 View

I am purifying one protein (which don't have any tag) by ammonium sulfate precipitation method. I am getting nonspecific bands after dialysis, why?

The saturation of ammonium sulfate precipitation is 50%, then ran SDS PAGE followed by Coomassie blue staining, i got nonspecific bands at different sizes along with my protein of interest. Can...

27 January 2025 3,182 3 View