Sitara Nasar

3 Questions 8 Answers 0 Followers

Questions related from Sitara Nasar

What is the need of performing native page gel? Does samples prepared with non-reductive dye without heating, run on SDS-PAG give the same insight?

My target protein exist in three conformations i.e. monomeric, dimeric and hexameric and I want to analyze them through gel electrophoresis. I want to ask should I perform native PAGE to get the...

03 March 2019 5,260 7 View

How can we confirm isolated viral RNA from serum sample?

Is RNA electrophoresis a best solution? If yes can anyone guide me the optimized protocol to perform RNA electrophoresis?

10 October 2018 9,868 4 View

Why there is a significant expression of a genetic fusion in pET-28a but not at all in pET-21a?

I have designed a genetic fusion of two separate constructs. One of them was in pET-21a and the other one was in pT7/7. Both of them expressed significantly in their respective plasmids but when I...

10 October 2018 7,313 4 View