Sipan Zhang

2 Questions 7 Answers 0 Followers

Questions related from Sipan Zhang

Does anyone know how to stably overexpress a microRNA in primary murine T cells whit high efficiency?

I've tried lentivirus but didn't get enough infected cells. An article (DOI:10.1172/jci78753) describes the following method: "B6 WT splenocytes were cultured in complete medium for 24 hours. The...

12 December 2015 2,989 5 View

Should we overexpress mouse gene or the human homologous gene in mice to prove its function when we found the later is functional in mice?

One miRNA was found to increase in patients with a certain disease. In former experiments we found that intravenous injection of the human miRNA induced mouse injury like the human disease. We...

08 August 2015 6,064 9 View