Does anyone know how to stably overexpress a microRNA in primary murine T cells whit high efficiency?

I've tried lentivirus but didn't get enough infected cells. An article (DOI:10.1172/jci78753) describes the following method: "B6 WT splenocytes were cultured in complete medium for 24 hours. The...

18 December 2015 1,422 4 View