Sinem Karaman

4 Questions 19 Answers 0 Followers

Questions related from Sinem Karaman

How to write a simple imageJ macro to measure signal intensity in manually selected ROI and manually set thresholds?

Hi Everyone, I would like to generate a very simple ImageJ macro, which first measures the area in a user-selected region of interest (ROI) and then the fluorescence signal intensity within the...

04 April 2018 7,941 2 View

Any suggestions on which phospho-IRS-1 antibody to purchase for western blots?

I would like to check in vivo insulin action, by assessing the IRS-1 and phospho-IRS-1, together with Akt and phospho Akt in adipose tissue after insulin administration. Any suggestions on...

05 May 2013 4,338 10 View

Designing FRET (Taqman) probes for alternative splicing: considerations?

We would like to design FRET type of probes to detect the presence of alternatively spliced isoforms of certain genes. My question is regarding the optimal probe design. How long the probe should...

01 January 2013 9,554 1 View

If you had steady state gene expression data, how would you express it?

I have cDNA from isolated murine macrophages (I have 4 samples from different mice) and I wanted to check the gene expression of 5 different genes. I obtained Ct values for these genes, however I...

10 October 2012 8,198 20 View