Sinem Yilmaz

2 Questions 2 Answers 0 Followers

Questions related from Sinem Yilmaz

What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

After the transfection, I blotted the membranes with commonly used beta-actin ab (sigma) in our lab. I saw 2 bands for the fırst time and I do not know the reason. Thank you for your concern. Sinem

26 December 2020 8,819 3 View

How can I solve my western problem related to image quality?

After completing all steps including transfer, blotting and antibody staining, images obtained were as blurry. I added an image for an example. How can I increase the image quality? Is it related...

13 July 2016 8,817 11 View