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Questions related from Sindhushree Raghunandan
I am interested in the oxidative stress response in ECs and I have noticed that after treatment with H2O2, cells are going into cell cycle arrest and are down-regulating DNA replication and repair...
11 August 2017 9,620 2 View
I have three biological repeats of my experiment but the variance across the experiments is very high (although the trend among my treatment groups in each experiment is consistent). Does anyone...
09 August 2017 7,367 3 View
I'm seeing up-regulation of both pro- and anti-apoptotic genes upon oxidative stress in endothelial cells; it's a very strong trend over a 16 hour time-course - not sure how to explain this. Any...
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I'm trying to find an unbiased quantitative approach for analyzing beta-galactosidase staining. If there is no other way than to manually count, what is the recommended approach? how many cells...
04 December 2014 5,534 4 View
Does anyone have recommendations of recent papers or reviews that shed light on the role of metabolism (i.e metabolic processes or pathways) in the development of senescence (either replicative or...
20 August 2014 9,493 3 View
After the SDS-Page gel was complete, the sample was transfered onto a PVDF membrane using Thermo Scientific's Semi-Dry transfer bed. Then, after primary and secondary staining, the membrane is...
06 May 2013 6,865 5 View