Sindhushree Raghunandan

6 Questions 8 Answers 0 Followers

Questions related from Sindhushree Raghunandan

Endothelial Cells: Cell Cycle Arrest, DNA Damage, and Senescence

I am interested in the oxidative stress response in ECs and I have noticed that after treatment with H2O2, cells are going into cell cycle arrest and are down-regulating DNA replication and repair...

11 August 2017 9,622 2 View

Statistical Analysis of Agilent Seahorse MitoStress Test Data

I have three biological repeats of my experiment but the variance across the experiments is very high (although the trend among my treatment groups in each experiment is consistent). Does anyone...

09 August 2017 7,369 3 View

Has anyone seen up-regulation of both pro- and anti-apoptotic genes in RNAseq data?

I'm seeing up-regulation of both pro- and anti-apoptotic genes upon oxidative stress in endothelial cells; it's a very strong trend over a 16 hour time-course - not sure how to explain this. Any...

18 June 2017 7,594 4 View

Is there an automated way to quantify beta-gal positive cells without manually counting?

I'm trying to find an unbiased quantitative approach for analyzing beta-galactosidase staining. If there is no other way than to manually count, what is the recommended approach? how many cells...

04 December 2014 5,536 4 View

Metabolic drivers and regulation of senescence?

Does anyone have recommendations of recent papers or reviews that shed light on the role of metabolism (i.e metabolic processes or pathways) in the development of senescence (either replicative or...

20 August 2014 9,495 3 View

What is the best way to quantify protein fold change from a western blot using ImageJ?

After the SDS-Page gel was complete, the sample was transfered onto a PVDF membrane using Thermo Scientific's Semi-Dry transfer bed. Then, after primary and secondary staining, the membrane is...

06 May 2013 6,867 5 View