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Questions related from Sinan Baho
I treated my RNA sample with DNase and I measured DNA concentration using Nanodrop and I found it was 2.28ng/µl. can I do cDNA reaction to perform qPCR reaction with this small amount of...
18 June 2018 1,269 8 View
I am trying to design an experiment to delete LuxS gene from antibiotic resistant E. coli strain CTX-M-15 but I cant use plasmid with Kanamycin because this strain is resistant to Kanamycin, is...
02 November 2017 3,562 4 View
I am using RNeasy plus mini kit for extraction of RNA from E. coli. I am following the procedure as written but when I run my eluted sample on the agarose gel to see the RNA integrity (which...
01 November 2016 5,553 7 View
during the growth curve experiment, why sometimes we get a new log phase during the stationary phase of the growth?
13 February 2016 8,793 2 View
Im preparing E. coli and Klebsiella pneumoniae samples for SEM and I read about ethanol series but no one explain it in details. How long shall I put each sample in the series?
03 January 2016 9,188 2 View
I am using a CDC biofilm reactor, I need to determine the residence time. Shall I choose it randomly or there is an equation depending on the doubling time? my strains that I am working with are...
12 December 2015 5,215 7 View
