Sina Khorsand

3 Questions 6 Answers 0 Followers

Questions related from Sina Khorsand

My protein of interest is aggregating / precipitating out during purification. Need some advice

Hello Everyone, So I usually purify hexahistadine KRas (1-166) through BL21-A1 expression system. This is the truncated g-domain of the protein. After affinity purification, I run it through FPLC...

12 June 2017 9,890 16 View

His-Tagged flavoprotein will not elute from Ni column. Should I optimize my buffer?

After having trouble expressing a prokaryotic flavoprotein due to inclusion body formation, we resorted to SF9 insect cell expression. We were able to finally obtain full length, soluble protein....

06 October 2014 2,034 3 View

How can we avoid inclusion body formation in SF9 insect cell expression?

We are expressing a fluorescent prokaryotic protein, and have had lots of trouble expressing the full length protein in E.coli without inclusion body formation (tried different expression...

19 September 2014 7,873 2 View