Trouble cloning a 1920 bp insert into a 7767bp vector. Why am I not getting any colonies after transformation?

I have to clone a 1920 bp insert cut with NheI and HIndIII into my 7767bp vector cut with the same enzymes. Protocol used: 1. I cut 5 ug of insert vector and 2 ug of destination vector and gel...

22 February 2024 3,287 3 View