For one-to-one Y1H expt (CloneTech Takara) should we include stop codon while designing primers for prey cDNA isolation(for cloning in pGADT7) or not?

Dear All We are planning to conduct one-to-one yeast one hybrid experiments. The bait (promoter) is cloned in pAbAi vector and the prey (Transcription factor) is to be cloned in pGADT7. I am...

05 April 2019 2,532 2 View