Simone Castellana

2 Questions 3 Answers 0 Followers

Questions related from Simone Castellana

Loading Dye in Q5 PCR product?

Hi everyone, I have a question that might sound silly. I'm performing a PCR with Q5 polymerase, in a reaction volume of 25mL. Now, I would need to run all of the volume of the reaction to excise...

15 July 2021 7,443 1 View

Smear with Phusion PCR, any suggestion?

Hi everyone, I have been struggling with a PCR amplification for weeks now. First, let me explain to you the context: I'm amplyfing a gene for a subsequent cloning (the gene is 2.5kb,...

24 June 2021 9,755 3 View