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Questions related from Simona Mura
I would like to stably transfect the Huvec cells with the GFP or RPF. Any advice or protocol to suggest?
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I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...
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I wish to prepare cryosections of spheroids, do you have any protocol for OCT embedding (How many spheroids? how keep in the center of the holder, etc)? could you suggest me also some providers...
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I need to perform some experiments with HEPG2 cells in hypoxia conditions. I have a doubt on the mixture of gas we have to use. Is the mix 1%O2/94%N2/5%CO2 correct? or should I work in complete...
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