Simona Mura

4 Questions 11 Answers 0 Followers

Questions related from Simona Mura

How can I stably transfect HUVEC cell line with the GFP?

I would like to stably transfect the Huvec cells with the GFP or RPF. Any advice or protocol to suggest?

07 July 2016 2,002 1 View

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

07 July 2016 1,626 2 View

Could you suggest any spheroids cryosectioning protocol?

I wish to prepare cryosections of spheroids, do you have any protocol for OCT embedding (How many spheroids? how keep in the center of the holder, etc)? could you suggest me also some providers...

01 January 2016 1,712 3 View

1% Oxygen for hypoxia experiments?

I need to perform some experiments with HEPG2 cells in hypoxia conditions. I have a doubt on the mixture of gas we have to use. Is the mix 1%O2/94%N2/5%CO2 correct? or should I work in complete...

02 February 2015 4,680 8 View