Simona Mura

3 Questions 5 Answers 0 Followers

Questions related from Simona Mura

Fibronectin antibody clone FBN11?

I wish to stain fibronectin in 3D tumor spheroids made with human cancer cells and fibroblasts. do you have a good antibody to suggest? Have you ever used the references MA5-11981 from...

01 July 2016 2,027 2 View

Could you suggest any spheroids cryosectioning protocol?

I wish to prepare cryosections of spheroids, do you have any protocol for OCT embedding (How many spheroids? how keep in the center of the holder, etc)? could you suggest me also some providers...

07 January 2016 10,017 4 View

How dissociate pancreatic cancer cells clusters with collagenase? Which concentration is used??

I'm preparing pancreatic cancer cells spheroids. Which concentration of collagenase can be used to dissociate them?

07 January 2016 7,809 3 View