Simon Sattler

2 Questions 3 Answers 0 Followers

Questions related from Simon Sattler

Why do I see a smear in my SDS gel after staining with instant blue?

At the moment, I'm struggling with a very basic technique. I want to run proteins from lysed T-REx HEK293 cells on a SDS gel. However, instead of sharp protein bands I just see a smear after...

25 September 2015 2,251 10 View

Why don't I see a band of my FLAG tagged protein in western blots?

I have constructed two versions of my GOI with and without a FLAG tag and cloned them into the same vector. However, when I transfect HEK293 cells with my two different constructs, I can detect my...

26 March 2014 485 15 View