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Hey all! I am wondering what the minimum requirements for a plasmid are. I am fully aware of all the required parts (i.e. ori, selection marker, promoter for expression and so on), but I am...
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Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....
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