4 Questions 13 Answers 0 Followers
Questions related from Simon Flueckiger
Hey all! I am wondering what the minimum requirements for a plasmid are. I am fully aware of all the required parts (i.e. ori, selection marker, promoter for expression and so on), but I am...
18 April 2023 4,533 4 View
Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....
27 August 2020 9,653 1 View
Dear all, I am growing genetically modified S. typhimurium SL1344 cells in a chemically defined medium in a bioreactor. The cells have a deletion in the aroA gene, rendering them auxotrophic...
11 December 2019 2,580 3 View
Im am planning to do some BiFC Assays, in N. benthamiana and onion. And im wondering what kind of controls i should include. Ideally as a positive control, I would use a gene pairs that is known...
12 July 2017 8,190 3 View
