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I have to use TMAO as a supplement in my expression media to check if it affects the protein expression in any way. So for that, can I autoclave it or filter sterilize TMAO?
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I have performed restriction digestion with ScaI for plasmid DNA. By insilico analysis, I saw that there is only one site for ScaI in my plasmid. So in normal conditions, I should get only one...
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