Simant Kumar

2 Questions 6 Answers 0 Followers

Questions related from Simant Kumar

Can I autoclave trimethylamine N-oxide or filter sterilization is safer option?

I have to use TMAO as a supplement in my expression media to check if it affects the protein expression in any way. So for that, can I autoclave it or filter sterilize TMAO?

23 November 2021 4,687 0 View

Restriction Digestion with ScaI ?

I have performed restriction digestion with ScaI for plasmid DNA. By insilico analysis, I saw that there is only one site for ScaI in my plasmid. So in normal conditions, I should get only one...

13 May 2020 5,128 11 View