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Questions related from Silvia Napolitano
Good morning, I am purifying a thioredoxin mutant (but behaving wt-like) from coli, with its unique Proline labelled for NMR (13C and 15N). On Friday I lysed the cells and collect the soluble...
11 September 2017 5,516 5 View
Dear all, I have found a pretty cute contamination in a colleague flask. We think it is a fungus, does anyone know which species it is (round, white and hairy) Have a nice fluffy day!
14 August 2017 6,096 7 View
Good morning everybody. I am purify MsrA from E. coli. I first run an anion exchange and now I am running a Phenyl sepharose (HIC). the buffer I am using is 20mM MOPS pH7 1.2M Ammonium sulphate...
30 May 2017 7,953 5 View
Good morning. I have two mutants of the same protein that differs one from the other by only one aminoacid (Leu/Ala). Unfortunately the samples where partially mixed and I have now a mixture of...
03 November 2016 1,038 6 View
I everybody! I am puryfing thioredoxin (extremely stable protein) and the last step is GF, in Sodium phosphate pH7 50mM. I will use this protein for Crystallization and spectroscopic...
27 October 2016 4,763 5 View
I am going to do a native-PAGE to see if my multisubunits protein (human RNase H2) is purified as a complex or if it breaks apart during NiNta purification. I would like to know if the conditions...
12 November 2015 1,756 3 View
