Any tips for primer designing or PCR conditions using PrimeSTAR GXL Polymerase?

Hi, I've been trying amplify a whole gene (44kbp) using PrimeSTAR GXL. The size of target is about 22kbp ( 2 parts). I designed primers using primer Blast. I chose primers of 30bp, single target ,...

12 December 2018 7,825 15 View