Siham Bibi

2 Questions 7 Answers 0 Followers

Questions related from Siham Bibi

Is it possible to detect phospho-protein but not total-protein using western blot?

I am looking for phospho-KIT (Y719) and total-KIT on mast cells using western blot.I can detect beautiful bands with phospho-KIT but it is impossible to detect total-KIT whatever I do! After...

06 June 2016 5,826 14 View

Why do my HMC-1 cells no longer express phospho-STAT5?

Since January, I'm not able to see any phosphorylation of both AKT, STAT3 and STAT5. Even when I try western blotting or phospho flow cytometry. I'm sure that there is no problem with, -...

07 July 2014 5,482 4 View