Sigrid Berg

4 Questions 3 Answers 0 Followers

Questions related from Sigrid Berg

How to calculate qualitative ELISA results?

I have done a qualitative ELISA-analysis, and I need some help with my calculations. I tested my protein against different receptors. I used a plate reader for the absorbance (450nm) of the wells,...

06 June 2019 2,796 2 View

How to calculate qualitative ELISA results?

I have done a qualitative ELISA-analysis, and I need some help with my calculations. I tested my protein against different receptors. I used a plate reader for the absorbance (450nm) of the wells,...

02 June 2019 5,181 2 View

Why are the bands from SDS-PAGE looking different than what I expected?

Hi, I´m doing recombinant expression of a scFv with a his-tag in E.coli BL21-AI with T7-promoter. The expression is induced by arabinose. I separated inclusion bodies and supernatant after...

23 May 2019 219 3 View

Why are the bands from SDS-PAGE looking different than what I expected?

Hi, I´m doing recombinant expression of a scFv with a his-tag in E.coli BL21-AI with T7-promoter. The expression is induced by arabinose. I separated inclusion bodies and supernatant after...

05 May 2019 2,049 3 View