Siew Wei Seah

3 Questions 3 Answers 0 Followers

Questions related from Siew Wei Seah

How to compare qPCR results with transcriptome results?

Hi everyone, I would like to do correlation analysis qPCR (2^(-delta delta Cq)) vs RNA-seq data in terms of fold change. In my qPCR, I have two conditions and one housekeeping genes to be...

19 September 2017 1,871 4 View

Overlapping genes detected by Cuffdiff as a single gene?

Hi, everyone! I have been using Cuffdiff to determine differential gene expression in Serratia marcescens. I notice that multiple genes shared the same transcript, giving the same FPKM values...

27 February 2017 9,679 3 View

Does Fireball RNA=tRNA?

Attached is my total RNA gel picture. Does anyone know that what is the fireball at the bottom of the gel? Is it symbolize degradation? or small RNA? On the other hand, I did once DNase treatment...

11 August 2016 8,266 5 View