Siddharth Singh | Jawaharlal Nehru Centre for Advanced Scientific Research

Siddharth Singh

3 Questions 5 Answers 0 Followers

Questions related from Siddharth Singh

How to normalize the expression level of our protein of interest while using two different cell lines?

Especially in the case when the loading control used shows different expression in different cell line.

12 December 2017 7,487 3 View

Can anyone suggest a standardized protocol for Ammonium sulphate precipitation with special emphasis on buffers used and their pH?

Mol wt - 29 kDa

03 March 2015 4,902 2 View

What are the ways by which basal level expression of the desired protein can be reduced in a T7 promoter driven expression of gene of interest?

Protein is non toxic to bacterial cells

02 February 2015 6,308 6 View