Kanamycin plasmid containing Ecoli(DH5a) are tough to break. Can anyone please suggest where should I look into?

I'm doing miniprep in LB broth for isolating pET28a plasmid, plasmid is resistant to kanamycin,  native DH5a cells are not growing on kanamycin plate. transformed cells are growing on LB plate,...

07 July 2017 2,921 4 View