SiCong Liu

2 Questions 2 Answers 0 Followers

Questions related from SiCong Liu

Can I use both spike-in (cel-miR-39) and endogenous RNA (U6) for normalization?

I am detecting ~15 different miRNAs in serum samples. For normalization it seems most people use spike-in (cel-miR-39) or endogenous small RNA. I see various studies normalize to multiple...

06 June 2016 2,978 2 View

How to improve poor quality of total RNA from miRNeasy serum/plasma kit?

Hi, I am extracting miRNA from serum samples with miRNeasy serum/plasma kit. From 200uL of starting serum, I get 15 - 20 ng/uL in 14 uL elution volume. This is low but still not bad. The main...

12 December 2015 1,079 5 View