Sibonelo Khumalo

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Questions related from Sibonelo Khumalo

Which method or procedure works best to generate C. elegans males?

I am trying to generate sufficient males for CB1003(kynu-1) strains by heat-shocking 10 L4 hermaphrodites at 30 degrees for 4 hours. However, after three days of incubation at 20 degrees, still I...

04 May 2021 4,120 5 View

Which method/procedure is more efficient to isolate DNA from a single worm?

I have been trying to confirm the genotype of the uaDf5 mutant C. elegans strain, however I keep on getting undesirable results. Is this due to difficulties in preparing the crude worm lysate(i.e....

23 March 2021 3,681 2 View