Shyamashree Banerjee

4 Questions 10 Answers 0 Followers

Questions related from Shyamashree Banerjee

How to interpret difference plot in HRM if the samples are going beyond 100%?

I am currently working on the epigenetic (methylation) aspect of coronary artery disease. After short-listing a list of CpG sites with the help of microarray data I am currently working on HRM to...

06 June 2019 5,599 3 View

How to validate methylation levels in HRM analysis?

Hello All, So, I am validating my Microarray results by HRM (High resolution melting) analysis. The CpG sites obtained by me are both hypo and hypermethylated. In the process of running my...

05 May 2019 6,003 3 View

In COBRA(Combined Bisulfite Restriction enzyme analysis) is it necessary to carry out electroblotting or after PAGE methylation % can be calculated?

I am working on methylation analysis of DNA (from blood/PBMC's). The technique which I will carry out is COBRA which is done by bisulfite conversion, followed by PCR amplification. Then based on...

10 October 2016 8,577 4 View

How does the confounding factors like Diabetes, HTN, drug intake affect methylation level?

How different should cases be in comparison to controls? I was wondering if confounding factors like Diabetes, HTN, lipid lowering drugs affect the methylation status of the individual.

05 May 2015 8,545 9 View