Shyamashree Banerjee

7 Questions 11 Answers 0 Followers

Questions related from Shyamashree Banerjee

What is the cut-off to determine the methylation level (hypo and hypermethylation) in Pyrosequencing?

Dear All, I am trying to validate my Microarray data (450k chip) using Pyrosequencing. The biggest challenge is how to designate the CpG sites as hypomethylated or hypermethylated? Is there a...

04 August 2020 9,253 0 View

How to interpret difference plot in HRM if the samples are going beyond 100%?

I am currently working on the epigenetic (methylation) aspect of coronary artery disease. After short-listing a list of CpG sites with the help of microarray data I am currently working on HRM to...

11 June 2019 1,168 3 View

In COBRA(Combined Bisulfite Restriction enzyme analysis) is it necessary to carry out electroblotting or after PAGE methylation % can be calculated?

I am working on methylation analysis of DNA (from blood/PBMC's). The technique which I will carry out is COBRA which is done by bisulfite conversion, followed by PCR amplification. Then based on...

03 October 2016 3,035 3 View

Should the population of my subjects have to have same inclusion/exclusion criteria if I want to replicate my Epigenetics Microarray results?

I want to replicate my Microarray (methylation) results in a larger population using a different technique. Should the population chosen for Microarray be the same for the replication population?...

30 May 2015 4,848 8 View

In Microarray , can I pool in DNA samples for methylation study?

I was planning for Methylation study using Microarray. If I want to pool in DNA samples what all should I keep in mind? Also, I have learnt that there might be some hindrance in the analysis of...

05 May 2015 3,536 2 View

Does anyone have a suggestion for techniques to use (other than Pyroseq) to validate a CpG site? Which is more sensitive; MSP or COBRA?

I am aware that Pyro is the gold standard to validate a methyaltion study. However, it has restrictions like it can read up to 70-100 bp only. So, if I want to do Methylation specific PCR which is...

11 April 2015 3,574 10 View

Methyaltion extent of CpG sites by HRM analysis

I am currently working on HRM analysis of individual CpG sites. I am trying to understand the extent of methylation (%) in cases against controls. However, the derivative melt curve analysis is...

01 January 1970 7,000 1 View