Shyam Kishor Sah

5 Questions 7 Answers 0 Followers

Questions related from Shyam Kishor Sah

Visualisation of protein produced by topically applied engineered bacteria on 3D skin surface?

Can we visualize protein produced by topically applied engineered bacteria using 3D skin model? I plan to topically apply the engineered bacteria on the3D skin model and visualize distribution...

06 July 2023 1,650 0 View

Does anyone have working protocol for immunostaining of collagen-based 3D skin equivalent?

Does anyone have experience of fixing 3D skin construct with 4% PFA and then embedding in cryomatrix for cryo-sectioning? What is the best method to fix and embed such constructs for histological...

16 April 2021 6,540 2 View

Do I need cardiac perfusion for spleen cell staining (IHC)?

Actually I am asked to stain neutrophils, dendritic cells, T cells in mouse spleen. Do I need to do cardiac perfusion for it?

01 July 2015 524 7 View

How can I make cell suspension from mouse skin for flow cytomteric analysis of immune cells population?

I need to evaluate the % of T cells, neutrophils and dendritic cells in skin sample by flow cytomtery. I would really appreciate any suggestions for making effective cell suspension from the skin....

26 May 2015 5,801 2 View

Visualisation of protein produced by topically applied engineered bacteria on 3D skin surface

Hi all, Can we visualize protein produced by topically applied engineered bacteria using 3D skin model? I plan to topically apply the engineered bacteria on the3D skin model and visualize...

01 January 1970 7,526 0 View