Shweta Kishen

2 Questions 2 Answers 0 Followers

Questions related from Shweta Kishen

Why nothing is visible after Site Directed Mutagenesis-PCR product's DpnI digestion?

I am doing site directed mutagenesis using stratagene-QuikChange II Site-Directed Mutagenesis Kit. The protocol and primer design I'm following are given in the link below. I am not getting any...

02 August 2021 846 9 View

Why do I get no colonies on transformation after SDM?

I am doing Site directed mutagenesis using QuikChange™ site-directed mutagenesis kit. The primers designed and protocol followed were as per "An efficient one-step site-directed deletion,...

14 May 2021 6,337 3 View