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Questions related from Shubham Sharma
We generally use. E.coli BL 21 for protein expression and E.coli DH 5a for cloning (amplification). But why can't we use BL 21 for both cloning and expression both? That would save a whole lot of...
10 October 2017 2,524 6 View
I want to express a human protein in mammalian cells. Why it is always advised to first clone (amplify) it in bacteria and then use those cloned plasmids for expression in mammalian cells ? Can't...
18 September 2017 4,406 11 View