Shubham Sharma

2 Questions 4 Answers 0 Followers

Questions related from Shubham Sharma

Why can't we use E.coli BL21 for both cloning (amplification) and expression?

We generally use. E.coli BL 21 for protein expression and E.coli DH 5a for cloning (amplification). But why can't we use BL 21 for both cloning and expression both? That would save a whole lot of...

10 October 2017 2,524 6 View

Why to use bacteria first to clone the gene of interest and then mammalian cells to express it ?

I want to express a human protein in mammalian cells. Why it is always advised to first clone (amplify) it in bacteria and then use those cloned plasmids for expression in mammalian cells ? Can't...

18 September 2017 4,408 11 View