Shu Liu

2 Questions 3 Answers 0 Followers

Questions related from Shu Liu

A very simple Cloning, but no colonies?

Dear all, I am cloning N-terminal GFP with Linker-MCS exchanging with C-terminal GFP in PBCAG-eGFP (addgene 40973) vector, the enzymes I used are EcoRI/NotI. This sounds like a very simple...

03 December 2021 1,312 4 View

Is there a good gold-labeled HA antibody?

I would like to stain my HA-tag fused NM protein for EM. Did anybody have experience with good gold-labeled HA antibody, or a good combination with primary and secondary gold-labeled HA antibody....

15 January 2015 3,242 2 View