I am unable to amplify a 1651 bp fragment from my plasmid using Taq Polymerase ? How do I troubleshoot this ?

I am using a plasmid (whose identity I confirmed by restriction digestion) for amplifying a 1651 bp amplicon using Taq Polymerase. The primers anneal around 20 bp on template, and are 40% GC....

05 March 2025 4,293 5 View