Can I keep the primary culture of E. coli at 4 degrees for 2-3 hrs before inoculating the secondary culture for protein overexpression?

Due to time constraints, I can’t seem to keep the secondary culture for 16 hr incubation (after induction) at any time so, I’m thinking of delaying inoculation with the primary culture. Is this...

04 March 2025 2,617 1 View