Shreeya Ravisankar

4 Questions 5 Answers 0 Followers

Questions related from Shreeya Ravisankar

Will a flavonoid linked to a sugar with a methyl group elute before a flavonoid acyl linked with a sugar?

Will O-methyl-chrysoeriol-7-O-rhamnosyl-glucosyl-glucoside elute before Chrysoeriol-7-O-coumaroyl-glucosyl-glucoside on a C18 reverse phase column analyzed with UPLC-MS/MS methodology?

05 May 2019 4,345 10 View

Why mass spectroscopy has sensitivity differences between flavonoid compounds with different groups attached?

Hello I am trying to detect flavonoids - quercetin and its corresponding O-glucuronide (metabolite) form on a UPLC-TQD mass spec instrument using MRM (multiple reaction monitoring) mode. The...

02 February 2019 9,403 3 View

Direct protein estimation from cultures cells in a 96 well plate?

I want to measure protein levels of cells attached to a 96 well plate. If the lysis buffer is added to the plate itself, is it alright if the cell debris remain in the plate (if any remain after...

08 August 2016 4,745 2 View

Is it alright to dissolve sample in DMSO to inject in an ESI-TQD mass spec?

The injection volume of the sample is just 2uL. The sample does not dissolve well in water/100% methanol/or mixture of water-methanol. The sample dissolves well in DMSO.

07 July 2016 4,055 2 View