Is PMA/Inomycin/BrefaldinA stimulation for 4-6 hrs enough to stimulate IFNg, Il-13, IL-17A and Il-10 from mouse splenocytes and lung cells?

Hello Everyone, I am trying to analyse cytokine production from lung cells and splenocytes from infected and uninfected (naive) mice for FACS analysis. As of now, I tested splenocytes and lung...

04 August 2017 3,163 4 View