Shradha Surin

2 Questions 2 Answers 0 Followers

Questions related from Shradha Surin

What parameters do I change to get binding of proteins in affinity chromatography?

I have been purifying His-tagged protein using Ni-NTA beads. I have been successful in eluting the wild type protein. Elution buffer constituents are: Tris 50mM, KCl 0.5M, Imidazole 500mM....

30 August 2023 2,942 3 View

I am getting smears in EMSA (electrophoretic mobility shift assay) result. what should i do to show proper binding instead of smear??

My EMSA results show smears of DNA protein complex instead of band, maybe because of non stable protein DNA complex. I tried reducing salt concentrations, but it didnt work. I have also tried...

31 January 2023 9,140 6 View