Shradha Surin

3 Questions 4 Answers 0 Followers

Questions related from Shradha Surin

When designing primers for tagging a protein at N terminal, should we remove the start codon of the protein?

I need to flag-tag my protein at N-term, and while designing the primers i was confused whether to remove the start codon of the protein, falling immediately after the flag sequence ends and the...

24 October 2024 7,088 4 View

What parameters do I change to get binding of proteins in affinity chromatography?

I have been purifying His-tagged protein using Ni-NTA beads. I have been successful in eluting the wild type protein. Elution buffer constituents are: Tris 50mM, KCl 0.5M, Imidazole 500mM....

30 August 2023 3,176 3 View

I am getting smears in EMSA (electrophoretic mobility shift assay) result. what should i do to show proper binding instead of smear??

My EMSA results show smears of DNA protein complex instead of band, maybe because of non stable protein DNA complex. I tried reducing salt concentrations, but it didnt work. I have also tried...

31 January 2023 9,190 6 View