Shraddha Thakkar

4 Questions 2 Answers 0 Followers

Questions related from Shraddha Thakkar

What are the cytokines used for short-term in vitro culturing of sorted Leukemic stem cells from AML cell lines?

I wish to maintain the leukemic stem cell phenotype of sorted cells (FACS sorted). Can someone suggest any cytokines that can be added to RPMI medium for culturing these sorted leukemic stem cells...

20 July 2018 1,680 0 View

Can someone suggest a method for enzymatic dissociation of a subcutaneous tumor followed by preparing a single-cell suspension of it?

Hi! I wish to perform FACS of cells isolated from a subcutaneous tumor. Kindly suggest methods that would be suitable for making single-cell suspension of the tumor excised from mice

04 April 2018 5,952 2 View

What is the average time duration taken by Kasumi-1 (AML cell line) cells to form tumors after subcutaneous injection into NOD/SCID mice?

I have subcutaneously injected 5 million Kasumi-1 cells into NOD/SCID mice. I wish to know how long does it take to form tumors.

13 March 2018 1,073 2 View

Can we store propionylated and trypsinised histone samples before proceeding for Zip-tip and LC MS MS?

Hi! I happen to encounter a need to store my propionylated and trypsinised histone samples before proceeding for zip-tip processing of them. I am not sure whether we can store them. Please suggest.

02 November 2017 9,162 3 View