Shiying Liu

2 Questions 2 Answers 0 Followers

Questions related from Shiying Liu

Can I use immortalised MEF P4-P5 to be as feeder cell for mouse embryonic stem cell E14TG2a?

Hi, our lab normally uses feeder-free method to maintain E14TG2a, mES cell line. Recently, I am trying to thaw the ATCC E14TG2a new cell line, which recommends to be grown on feeder cell. Many...

02 February 2020 1,675 3 View

Does anyone know about isolation and enrichment of breast cancer stem cell by culturing MCF-7 in the low attachment surface?

Anyone knows about isolation and enrichment breast cancer stem cell by culturing MCF-7 in the low attachment surface? In the process to form into a tumorsphere, how often do you add new medium?...

11 November 2015 4,272 3 View