Shivani Gupta

2 Questions 4 Answers 0 Followers

Questions related from Shivani Gupta

How can I successfully use Gibson to assemble a small fragment (120bp) into a 4kb vector that must be gel-extracted?

I'm trying to insert a 120bp segment into a 4k vector using Gibson Assembly. The purpose is to replace a 27bp portion of the vector with NNKs. The insert is ordered as an ssDNA oligo, and then...

10 October 2014 9,431 3 View

In a dual-plasmid system, what E. coli cloning vector for moderate/high copy number would be compatible with a pUC vector?

I'm expressing fimbriae in two parts in my E. coli. Vector 1 contains everything except the tip (FimH), and Vector 2 contains FimH. Vector 1 is a pCRII-TOPO plasmid with ColE1 (pUC) origin of...

06 June 2014 4,311 5 View