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I'm trying to insert a 120bp segment into a 4k vector using Gibson Assembly. The purpose is to replace a 27bp portion of the vector with NNKs. The insert is ordered as an ssDNA oligo, and then...
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I'm expressing fimbriae in two parts in my E. coli. Vector 1 contains everything except the tip (FimH), and Vector 2 contains FimH. Vector 1 is a pCRII-TOPO plasmid with ColE1 (pUC) origin of...
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