Shivani Soni

2 Questions 7 Answers 0 Followers

Questions related from Shivani Soni

Not able to get the transformed colonies after performing ligation?

Hello everyone, I am not able to get the transformed colonies after performing ligation of the pET28a vector and HAV gene of intrested. I am using E coli. DH5alpha strain, but after transforming...

05 December 2024 9,993 8 View

Issues with Protein Precipitation During Alkaline Lysis for Plasmid Isolation while performing molecular cloning?

Hello everyone, I am currently working on molecular cloning. After preparing the ligation mixture, I transformed it into cells. The next day, I observed colonies on LB medium with kanamycin,...

30 November 2024 3,676 6 View