Has anyone experienced 100% iPS cell death 24 hrs after electroporation with plasmids (endotoxin-free and low endotoxin prepared)?

We have been unable to transfect stem cells using a protocol that has been in existence in our lab since 2014. Essentially all the cells that take up plasmid appear to die. This is what we are...

21 November 2019 5,092 2 View