Shivaksh Ahluwalia

3 Questions 4 Answers 0 Followers

Questions related from Shivaksh Ahluwalia

Which LncRNA transcript variant do I study?

The LncRNA I am interested in studying has many transcript variants, 14 as per ensembl. I want to study the effect of over-expression of this LncRNA. To do so I want to clone the LncRNA into a...

02 February 2018 5,248 6 View

Can someone please suggest a protocol for determining MOI for HBV?

I need to determine MOI of viral particles present in media post HepG2 transfection of HBV genome. I understand I will need to determine PFUs to calculate MOI. I would be grateful if someone can...

12 December 2017 3,306 2 View

How do we identify DEGs in tumour (disease) vs non-tumour (non-disease) samples?

In a dataset I am analysing, there is a single replicate of RNA seq (or microarray) data each for tumour tissue and adjacent non-tumour tissue. Such data is available for say 10 patients in the...

11 November 2017 4,985 8 View