Shivaani Srinivasan

3 Questions 6 Answers 0 Followers

Questions related from Shivaani Srinivasan

What could be the reason for Alpha-tubulin showing multiple bands and high background in western blot?

We used tumor and normal tissue lysate and an equal amount of 30 ug of protein was loaded to each well. The lysate was stored in -80 degree. After mixing the dye it was stored in -20 degree. Also...

27 November 2021 1,572 5 View

Could someone recommend me a protocol for tissue lysate preparation from freshly resected tumor samples?

Any articles or protocols related to the preparation of tissue lysates from freshly resected tumor samples for Western Blot would be appreciated

26 October 2021 2,182 3 View

Why is my DAB reagent forming clumps (Brownish black particles)? How do I resolve this issue?

I tried spinning the solution down for 5 minutes. I could see the particles settle down but I still saw these black particles in the tissue section.

04 August 2021 5,877 2 View