Shiv Kumar

4 Questions 5 Answers 0 Followers

Questions related from Shiv Kumar

What may be the reasons behind the shrinkage of RBC during the SEM sample preparation?

Dear researchers, I am facing a problem with the shrinkage of RBC during the SEM sample preparation. In brief, blood was collected from the fish, and the smear was prepared on a 10mm circular...

02 May 2024 7,245 3 View

How to estimate the concentration of a chemical compound in E3 media via HPLC?

I have to estimate the concentration of a chemical compound in E3 media used in zebrafish embryo exposure to that chemical. What should be the method or protocol for sample preparation for this ?

16 November 2023 9,737 0 View

What may be the reason behind the degeneration/degradation of 96h zebrafish larvae during the AO and DCFH-DA staining to study the apoptosis and ROS?

20-25 larvae (96h) were randomly selected, washed 2-3 times with PBS, and then stained with AO(5ug/ml) and DCFH-DA(10uM) stain for 30 min separately and then washed with PBS 2-3 times to remove...

28 July 2023 9,641 4 View

Is there any problem, if we design experiment for the determination of LC50 of a compound in Zebra fish during its breeding season?

Is the determination of LC50 of any compound in Zebra fish during the breeding season cause any problems?

05 July 2021 9,550 3 View