Shiv Kumar

5 Questions 5 Answers 0 Followers

Questions related from Shiv Kumar

What may be the reasons behind the shrinkage of RBC during the SEM sample preparation?

Dear researchers, I am facing a problem with the shrinkage of RBC during the SEM sample preparation. In brief, blood was collected from the fish, and the smear was prepared on a 10mm circular...

02 May 2024 7,305 3 View

How to estimate the concentration of a chemical compound in E3 media via HPLC?

I have to estimate the concentration of a chemical compound in E3 media used in zebrafish embryo exposure to that chemical. What should be the method or protocol for sample preparation for this ?

16 November 2023 9,785 0 View

What may be the reason behind the degeneration/degradation of 96h zebrafish larvae during the AO and DCFH-DA staining to study the apoptosis and ROS?

20-25 larvae (96h) were randomly selected, washed 2-3 times with PBS, and then stained with AO(5ug/ml) and DCFH-DA(10uM) stain for 30 min separately and then washed with PBS 2-3 times to remove...

28 July 2023 9,713 4 View

Is there any requirement of ethical permission for the research work on early stages of zebra fish upto 96 hours post fertilization?

We want to study the toxicity in early stages of zebra fish upto 96 hpf.

19 July 2021 6,407 2 View

Is there any problem, if we design experiment for the determination of LC50 of a compound in Zebra fish during its breeding season?

Is the determination of LC50 of any compound in Zebra fish during the breeding season cause any problems?

05 July 2021 9,618 3 View